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目的 了解一氧化氮 (NO)信息传递系统对呼吸道上皮细胞纤毛运动频率 (CBF)的影响。方法 选用雄性SD大鼠 9只 ,异氟醚麻醉后以无菌技术迅速取其气管 ,并将气管粘膜切成1mm2大小的组织块 ,在DMEM中进行组织培养。将组织块分成 5组 :L 精氨酸 (L Arg)组 ;1 Hy droxy 2 oxo 3 (N ethyl 2 aminoethyl) 3 ethyl 1 triazene (NOC 12 ,NO载体 )组 ;8 Br cGMP组 ;D Arg组及磷酸盐缓冲液 (PBS)组。以相差显微镜及成像分析技术测定CBF ,对纤毛运动进行定量分析。结果 (1)给L Arg后CBF由 (7 43+0 75 )Hz增至 (8 5 9+0 93)Hz(P <0 0 1) ;给NOC 12后CBF由 (7 6 6 +0 6 8)Hz增至 (8 6 2 +0 91)Hz(P <0 0 5 ) ;给 8 Br cGMP后CBF由 (7 11+0 6 6 )Hz增至(8 10 +0 83)Hz(P <0 0 5 ) ;(2 )PBS及D Arg对CBF无影响。 结论 NO信息传递系统能使大鼠呼吸道上皮细胞CBF加快。
Objective To investigate the effect of nitric oxide (NO) signaling system on ciliary movement frequency (CBF) in airway epithelial cells. Methods Nine male Sprague-Dawley rats were used. After isoflurane anesthesia, the trachea was rapidly removed by aseptic technique. The tracheal mucosa was cut into 1mm2 tissue pieces and cultured in DMEM. Tissues were divided into five groups: L Arg group; 1 Hy droxy 2 oxo 3 (N ethyl 2 aminoethyl) 3 ethyl 1 triazene group; 8 Br cGMP group; D Arg group And phosphate buffered saline (PBS) group. CBF was measured by phase-contrast microscopy and imaging analysis to quantitatively analyze cilia movement. The results showed that CBF increased from (7 43 + 0 75) Hz to (859 + 0 93) Hz (P 0 01) to L Arg, and to (76 6 + 0 6 (8 8 2 +0 91) Hz (P 0 05); the CBF increased from (7 11 + 0 6 6) Hz to (8 10 +0 83) Hz (P <0 0 5); (2) PBS and D Arg had no effect on CBF. Conclusion NO signaling system can accelerate the CBF of rat respiratory epithelial cells.