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目的 :测定 7种葛属植物根中异黄酮成分的含量。方法 :采用高效液相色谱法。色谱柱为HypersilODS2(15 0mm× 4 6mm ,5 μm) ;检测波长 2 5 0nm ;葛根素、大豆苷、大豆苷元的加样回收率±RSD分别为 (10 5 7± 4 6 ) % ,(10 5 4± 6 9) % ,(10 4 5± 9 2 ) %。结果 :葛属植物中 3种异黄酮成分含量差异较大 ,野葛、粉葛和峨嵋葛中含有的葛根素和大豆苷比其它几种葛属植物高 ,野葛中葛根素的含量最高 ;从液相色谱图中观察到不同产地野葛的图谱与其它种的葛属植物有明显区别。结论 :异黄酮成分可作为评价葛属植物质量的参考依据
Objective : To determine the content of isoflavones in 7 kinds of Pueraria plants. Method : High performance liquid chromatography. The column was HypersilODS2 (15 0mm × 4 6mm, 5 μm); the detection wavelength was 250 nm; the recovery of puerarin, daidzein and daidzein was ±105% (RS), respectively ((5 5 7 ± 4 6) %, ( 10 5 4± 6 9) %, (10 4 5± 9 2) %. Results: The content of three kinds of isoflavones in Pueraria plants varied greatly. Puerarin and daidzein contained in Pueraria lobata, Pueraria lobata and Pueraria lobata were higher than those in other varieties of Pueraria. The content of puerarin in Pueraria lobata was highest. From the liquid chromatograms, it was observed that the map of Kudzu from different habitats was clearly different from other species of Pueraria. Conclusion : Isoflavones can be used as a reference for evaluating the quality of Pueraria plants