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为了解立克次体在人群中的感染情况,我们于1989年采用斑点式酶免疫染色法对济南市和莒南县部分人群进行了血清学调查。 (一) 材料和方法 1.血清来源:收集济南市某工厂健康人血清216份,其中男87份,女129份,年龄在18～50岁之间,均为济南市长住人口。另收集莒南县流行性出血热恢复期病人血清115份(经间接免疫荧光检测血清抗体证实),患者均为农民。以上血清标本均为静脉采血,-20℃保存。2.立克次体抗原片和酶标记结合物及阳性对照血清均由军事医学科学院微生物流行病研究所提供。3.方法:在每张抗原片上滴加1∶40稀释的待检血清,37℃
In order to understand the infection status of Rickettsia in the population, we carried out a serological investigation of some populations in Jinan and Taonan counties in 1989 by using the spot-enzyme immunostaining method. (A) Materials and Methods 1. Serum Source: collected from a factory in Jinan, 216 healthy human serum, of which 87 were males and 129 females, aged 18 to 50 years old, are living in Jinan mayor population. Another collection of Junan County epidemic hemorrhagic fever patients with serum 115 (confirmed by indirect immunofluorescence serum antibody), patients are farmers. The above serum samples were venous blood, -20 ℃ preservation. Rickettsia antigens and enzyme-labeled conjugates and positive control serum were provided by the Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences. 3. Method: Add 1: 40 diluted serum to be tested on each antigen plate, 37 ℃