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目的观察Rb基因及其产物在骨肉瘤细胞中的作用及其对转录因子E2F1的影响。方法转染pRb质粒进入骨肉瘤细胞系UMR-106,用噻唑蓝(MTT)法检测转染前后细胞增殖的变化,用逆转录-聚合酶链反应(RT-PCR)检测转染前后E2F1 mRNA的表达变化,用Western blot检测转染前后E2F1蛋白的表达变化。结果转染pRb质粒的骨肉瘤细胞UMR-106与未转染pRb质粒者相比细胞增殖受到抑制。转染pRb质粒的骨肉瘤细胞UMR-106与未转染者相比,转录因子E2F1 mRNA和蛋白的表达均减弱。结论Rb能抑制骨肉瘤细胞的增殖,这种增殖抑制作用与Rb对转录因子E2F1的表达抑制有关。
Objective To investigate the role of Rb gene and its products in osteosarcoma cells and its effect on transcription factor E2F1. Methods The pRb plasmid was transfected into the osteosarcoma cell line UMR-106. The cell proliferation was detected by MTT assay before and after transfection. The mRNA expression of E2F1 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) The expression of E2F1 protein was detected by Western blot. Results The proliferation of osteosarcoma cells transfected with pRb plasmid UMR-106 was inhibited compared with that of untransfected pRb plasmid. The expression of E2F1 mRNA and protein in osteosarcoma UMR-106 transfected with pRb plasmid was weaker than that in untransfected cells. Conclusion Rb inhibits the proliferation of osteosarcoma cells. The inhibitory effect of Rb on the inhibition of E2F1 expression by Rb is related to the inhibitory effect of Rb on the expression of E2F1.