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以脱毒甘薯腋芽节段为包埋体 (种心 ) ,用质量浓度 3 %～ 5 %海藻酸钠 (加入MS培养基和 3 %白糖 )和 2 %～ 4%CaCl2 包裹 ,制备人工种子。最佳腋芽长度为 3mm左右的节段 ;最佳凝胶系统为 4%海藻酸钠和 2 %CaCl2 ;人工种子在MS琼脂培养基上萌发率达 66% ,在蛭石加少量MS液体培养基中萌芽率达 64 % ,人工种子在低温 (4± 1℃ )储藏一定时间后萌芽率明显下降
Artificial seeds were prepared by embedding the axillary buds of sweet potato into the embedding body (seed heart) with 3% -5% sodium alginate (MS medium and 3% sugar) and 2% -4% CaCl2. The optimal axillary bud length was about 3mm. The best gel system was 4% sodium alginate and 2% CaCl2. The germination rate of artificial seeds on MS agar was 66%. In vermiculite plus a small amount of MS liquid medium In the germination rate of 64%, artificial seeds at low temperature (4 ± 1 ℃) stored for a certain time after the germination rate decreased significantly