Effect of Regulation of HSV-tk Gene Expression and Tumor Killed Activity with a Single Tetracycline

• 作 者: WANG Qian DU Zhen-wu MA Qing-s
• 机 构: CentralLaboratory,DepartmentofUrinarySurgery,SchoolofPharmaceuticalSciences,DepartmentofPediatrics
• 出 处: 高等学校化学研究：英文版
• 发表日期: 2009年5期
• 关键词: 单纯疱疹病毒 HeLa细胞 TK基因 载体系统 细胞活性 四环素 调控 PCDNA3.1 HSV-tk gene Tetracycline HeLa cells
• 基金项目: Supported Partly by the Program of Jilin Provincial Science ＆ Technology Department, China（Nos.200705335 and 200504057） and the Program of Development and Reform Commission of Jilin City, China（No.200

To construct a single tetracycline-regulatable plasmid vector based on the double tetracycline-regulatable plasmid vector system for regulating HSV-tk gene expression so as to effectively kill HeLa cells. Two tetracycline operator(TetO2) was cloned into p