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目的 :探讨温阳散结中药的抗肿瘤机制 ,研究温阳散结中药诱导BEL 740 2细胞凋亡作用。方法 :采用细胞形态学、原位末端标记和流式细胞技术 ,观察温阳散结中药对BEL 740 2细胞的影响。结果 :BEL 740 2细胞经中药处理后 ,光镜下可见凋亡细胞的形态学特征性改变 ,TUNEL标记阳性 ,流式细胞仪分析DNA含量 ,在非凋亡细胞二倍体峰 (G1)之前细胞出现典型的凋亡细胞峰 (Ap峰 )。 0 0 0 5g·ml-1中药组和 0 0 1g·ml-1中药组发生凋亡的细胞数分别为 13 9%和 2 1 6 %。结论 :温阳散结中药可诱导BEL 740 2细胞发生凋亡 ,并有随浓度升高作用增强的趋势 ,实验结果表明 ,诱导肿瘤细胞凋亡可能是温阳散结中药治疗肝癌的作用机理之一。
Objective : To investigate the anti-tumor mechanism of Wenyang Sanjie traditional Chinese medicine, and to study the apoptosis of BEL 740 2 cells induced by Wenyang Sanjie Chinese medicine. Methods : Cell morphology, in situ end labeling and flow cytometry were used to observe the effect of Wenyang Sanjie Chinese medicine on BEL 740 2 cells. RESULTS: After BEL 740 2 cells were treated with traditional Chinese medicine, the morphological changes of apoptotic cells were observed under light microscope. The TUNEL marker was positive. The DNA content was analyzed by flow cytometry before the non-apoptotic cell diploid peak (G1). The cell shows a typical apoptotic cell peak (Ap peak). The number of apoptotic cells in the 0 0 0 5 g·ml-1 Chinese medicine group and 0 0 1g·ml-1 Chinese medicine group were 13 9% and 2 16%, respectively. Conclusion: Wenyang Sanjie traditional Chinese medicine can induce apoptosis of BEL 740 2 cells, and there is a tendency to increase with increasing concentration. The experimental results show that the induction of tumor cell apoptosis may be the mechanism of Wenyang Sanjie Chinese medicine treatment of liver cancer. one.