论文部分内容阅读
目的观察焦炉逸散物职业暴露人群尿液中1-羟基芘浓度水平与P53基因第5、第8外显子损伤的关系。方法选取某焦化厂作业工人239人作为研究对象,高效液相色谱法检测尿液1-羟基芘水平;实时荧光定量PCR扩增人群外周血淋巴细胞P53基因外显子5和外显子8,通过循环阈值推算损伤后扩增效率,间接计算损伤指数。结果炼焦车间和焦油车间作业人员尿液中1-羟基芘浓度高于备煤车间和甲醇车间作业人员尿液1-羟基芘浓度;尿中1-羟基芘校准浓度与P53基因第8外显子损伤指数存在正相关。结论焦炉逸散物职业性接触可致第8外显子损伤突变增加,而与第5外显子损伤突变关系不大。
Objective To investigate the relationship between urinary levels of 1-hydroxypyrene and the 5th and 8th exons of P53 gene in workers exposed to coke oven workers. Methods A total of 239 workers in a coking plant were selected as research objects. The level of urinary 1-hydroxypyrene was detected by high performance liquid chromatography (HPLC). Exon 5 and exon 8 of P53 gene in peripheral blood lymphocytes were amplified by real- The damage index was calculated indirectly by calculating the post-injury amplification efficiency through the cycle threshold. Results The concentration of 1-hydroxypyrene in urine of workers in coking workshop and tar workshop was higher than that of workers in coal preparation workshop and methanol workshop. The concentration of urinary 1-hydroxypyrene in urine and the exon 8 of P53 gene There is a positive correlation between injury indices. Conclusion Occupational exposure to coke oven fume may increase the number of exon 8 mutations, but not the number of exon 5 mutations.