论文部分内容阅读
目的:研究补肾活血防粘汤对中、重度宫腔粘连分离术(IUA)后患者官腔粘连组织中TGF-β1、CTGF表达的影响,探讨补肾活血防粘汤防治中、重度IUA的可能作用机制.方法:将90例接受宫腔镜下宫腔粘连分离术(TCRA)的患者随机分成对照组、西药组及治疗组,对照组行TCRA后立即宫腔内留置Foley导管并注入2 mL手术防粘连液;西药组在对照组基础上予补佳乐、黄体酮胶囊行人工周期治疗;治疗组在对照组基础上配合口服中药补肾活血防粘连汤,连续治疗3个月经周期后复查宫腔镜,取各组患者宫腔内膜组织行HE染色,免疫组化法检测TGF-β1、CTGF的表达量.结果:对照组TGF-β1、CTGF的表达量明显高于西药组和治疗组(P＜0.05),而治疗组中TGF-β1、CTGF的表达量明显低于西药组(P＜0.05)和对照组(P＜0.01).结论:补肾活血防粘汤可有效预防TCRA后宫腔再次粘连,其机制可能与抑制TGF-β1来下调CTGF表达,延缓纤维化的形成有关.“,”Objective:To explore the effect of Bushen Huoxue Fangzhan Decoction (补肾活血防粘汤,BSHXFZD) on expression of TGF-β1,CTGF in patients with IUA.Methods:The 90 women with moderate or severe IUA was divided into the control group,western medicine group and treatment group,all cases underwent TCRA operation.The cases in control group received intrauterine indwelling Foley catheter and antiblocking liquid injection 2 mL,the western medicine group took the IUD with progynova and progesterone capsules,while the treatment group was given BSHXFZD besides the control group.After 3 months,the effect was observed by hysteroscopy,and endometrial tissue of patients were obtained,and HE staining was used to detect the expression of TGF-1 and CTGF by immunohistochemistry.Results:The expression of TGF-β1,CTGF in control group is significantly higher than western medicine group and treatment group (P＜0.05),the expression of TGF-β1,CTGF in the treatment group significantly lower than western medicine group (P＜0.05) and the control group (P＜0.01).Conclusion:BSHXFZD can effectively prevent TCRA intrauterine adhesions again,and the mechanism may related to inhibit the expression of TGF-1,increase the expression of CTGF,and delay the formation of fibrosis.