【摘 要】
：
研究发现 ,焦硫酸钾与还原糖可发生焦糖化反应 ,且三硝基酚有显著提高其测定灵敏度的作用 ,摸索确定了最佳反应条件 ,建立了快速测定血糖的新分光光度法。还原糖质量浓度在 0
【机 构】
：
安徽技术师范学院,安徽技术师范学院 凤阳233100,凤阳233100
论文部分内容阅读
研究发现 ,焦硫酸钾与还原糖可发生焦糖化反应 ,且三硝基酚有显著提高其测定灵敏度的作用 ,摸索确定了最佳反应条件 ,建立了快速测定血糖的新分光光度法。还原糖质量浓度在 0 .0 1～ 0 .0 5g/L范围内符合比耳定律 ,其λmax=46 0nm ,Sandell灵敏度为 48.6ng cm-2 ,Sr为 1.95 %～ 5 .97% ,R %为 97.0 %～ 10 5 .0 %。该法用于临床 ,所用试剂少 ,结果可靠。
The study found that potassium pyrosulfate and reducing sugar caramelization can occur, and trinitrophenol significantly increase the sensitivity of its role in exploring the best to determine the optimal reaction conditions, the establishment of a rapid determination of blood glucose spectrophotometry. The concentration of reducing sugar conformed to Beer’s law in the range of 0.010-0.5 g / L, λmax = 46 0 nm, Sandell sensitivity was 48.6 ng cm-2, Sr was 1.95% -5.97%, R% 97.0% ~ 105.5%. The law for clinical use less reagents, the results are reliable.
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