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胞浆内精子注射(ICSI)显著改善了由于男性不育造成的不育症的治疗,为了确定胚胎发育阶段对ICSI妊娠成功率的影响。作者对1994年1月～1995年10月455例ICSI治疗周期及304例传统的体外受精(IVF)治疗周期进行了回顾性分析。妇女年龄在≤40岁,行ICSI妇女至少有一次IVF失败或其配偶极度少精、弱精和/或畸形精子。 用于ICSI的精液为射出的精液或经皮附睾穿刺吸出的精子(PESA),用洗涤后上游法或Percoll梯度离心法分离精子。接受手术的妇女通过控制的超排卵刺激多卵泡发育,适时注射绒毛膜促性腺激素(hCG)后,经阴道取卵,将获取的卵子置于培养液中,在
Intracytoplasmic sperm injection (ICSI) significantly improves the treatment of infertility due to male infertility, in order to determine the effect of embryonic development on ICSI pregnancy success rate. The authors retrospectively reviewed 455 ICSI treatment cycles and 304 conventional IVF cycles from January 1994 to October 1995. Women aged ≤40 years, women with ICSI failed IVF at least one time or their spouse was severely weakened, weakened and / or deformed. Semen used in ICSI is sperm (PESA) aspirated semen or percutaneous epididymal puncture, and the sperm is separated by the post-wash upstream method or Percoll gradient centrifugation. Women who underwent surgery underwent controlled superovulation to stimulate the development of multiple follicles. After timely injection of human chorionic gonadotropin (hCG), vaginal ova were collected, and the obtained eggs were placed in a culture medium.