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建立了血液斑痕中格列本脲,格列美脲,格列吡嗪的液相色谱-质谱检验法.选用ACQUITY UPLC HSS C18(2.1×50 mm,1.8 μm)色谱柱,使用0.1％甲酸水-乙腈体系作为流动相进行梯度洗脱.采用ESI+电喷雾离子源,多反应监测扫描模式(MRM)检测3种磺酰脲类降糖药.在最优条件下,磺酰脲类降糖药在1.25 ～ 250 ng/patch (swab)范围内有良好线性关系,保留时间依次为2.90,3.00,2.34 min.检出限为0.1 ng/mL.渗透性客体上药物的回收率随着浓度的增大而增大,非渗透性客体上药物的回收率随着浓度的增大而减小.日内与日间精密度均小于15％,基质效应在0.85 ～ 1.15之间.可用于血液斑痕中格列本脲,格列美脲,格列吡嗪的快速筛查.“,”A liquid chromatography-mass spectrometry (LC-MS) method for the determination of glibenclamide,glimepiride and glipizide in bloodstains was established.The ACQUITY UPLC HSS C18 (2.1 ×50 mm,1.8 μm) column was eluted using a 0.1％ formic acid water-acetonitrile system as the mobile phase.The three sulfonylurea drugs were detected by LC-MS ESI + electrospray ionization source mode and multi-reaction monitoring scanning mode (MRM).Under the optimal conditions,the three sulfonylurea drugs had a good linearity in the range of 1.25 ～ 250 ng/patch (swab) with the retention time of 2.90,3.00 and 2.34 min,respectively.The detection limits were 0.1 ng/mL.The recovery of the drug on the permeable guest increasedwith the increase of the concentration,and the recovery rate of the drug on the non-permeable guest decreased with the increase of the concentration.Day and day precisionswere less than 15％ with the matrix effect between 0.85 ～1.15.This method can be used for rapid screening of glibenclamide,glimepiride,and glipizide in bloodstains.