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目的:本实验旨在探讨不同因素对小鼠受孕率的影响。方法:昆明系性成熟雌性小鼠分为4个实验组,每组40只,分别为2个12周龄组,1个9周龄组和1个12周龄用药组。1个12周龄组在春季合笼交配,另外3组均在夏季合笼交配,其中12周龄用药组给予腹腔注射HCG和PMSG。合笼时均以雌雄2:1交配。通过阴栓观察、阴道精子检查以及雌鼠体重的变化判断是否受孕,计算受孕率。结果:春季12周龄组的受孕率为17.5%,夏季12周龄组的受孕率为45%,夏季9周龄组的受孕率为0%,夏季12周龄用药组的受孕率为77.5%。在夏季组中,12周龄的受孕率为(45%)高于9周龄的受孕率为(0%)差异具有统计学意义(p<0.01);在12周龄组中,夏季组的受孕率为(45%)高于春季组的受孕率(17.5%),差异具有统计学意义(p<0.01);在12周龄夏季组中,用药组的受孕率为(77.5%)高于未用药组的受孕率(45%),差异具有统计学意义(p<0.01)。结论:12周龄雌鼠的合笼受孕率高于9周龄雌鼠,夏季组合笼受孕率高于春季组,用药组合笼受孕率高于未用药组。
Objective: This experiment aims to explore the different factors on the pregnancy rate of mice. Methods: Kunming lineage mature female mice were divided into 4 experimental groups, 40 in each group, which were 2 weeks old, 1 9 weeks old and 1 12 weeks old. One 12-week-old group was mated in the spring and the other three groups were mated in the summer. The 12-week-old mice were given intraperitoneal injection of HCG and PMSG. Cage were male and female 2: 1 mate. Through the vaginal plug observation, vaginal sperm examination and female body weight changes to determine whether the pregnancy, calculate the rate of pregnancy. Results: The conception rate of the 12-week-old group was 17.5%, the conception rate of the 12-week-old group was 45% in summer, the conception rate was 0% in the 9-week summer group, the conception rate was 77.5% . In the summer group, the conception rate at 12 weeks (45%) was higher than that at 9 weeks (0%) (p <0.01). In the 12-week-old group, The pregnancy rate (45%) was higher than that of the spring group (17.5%), the difference was statistically significant (p <0.01). In the 12-week summer group, the pregnancy rate was 77.5% The rate of pregnancy in non-medication group (45%) was statistically significant (p <0.01). CONCLUSION: The cage pregnancy rate of 12-week-old female rats was higher than that of 9-week old female rats. The cage pregnancy rate in summer group was higher than that in spring group, and the cage pregnancy rate in combination group was higher than that in non-medication group.