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探索研究纳豆菌液体发酵黄芩药材制备黄芩素和汉黄芩素的工艺,利用纳豆菌所产生的活性酶对黄芩苷和汉黄芩苷进行生物转化,以提高黄芩中黄芩素和汉黄芩素的含量。通过考察不同碳源、氮源及无机盐的种类和浓度、培养基p H、药材粉碎度、装液量、摇床转速、液料比、发酵时间等因素对发酵工艺的影响,以黄芩素和汉黄芩素的含量为评价指标,优选得到黄芩生物转化生成黄芩素和汉黄芩素的液体发酵最佳工艺为蛋白胨质量分数为1.0%,氯化钠0.05%,p H 6.0,黄芩药材粉碎过40目,装样量33%,摇床转速200 r·min~(-1),液料比5∶1,温度37℃,发酵6 d,黄芩药材中黄芩苷的转化率为97.6%,汉黄芩苷转化率97.0%。验证试验表明该工艺稳定可行,为黄芩素和汉黄芩素的工业化生产提供数据参考。
To explore the technology of baicalein and wogonin for the production of Scutellaria baicalensis Georgi by liquid fermentation of Bacillus natto, and to utilize the active enzyme produced by Bacillus natto to transform baicalin and baicalin so as to improve the bioavailability of baicalein and wogonin in baicalin content. The effects of various carbon sources, nitrogen sources and inorganic salts on the fermentation process were investigated. The effects of factors such as the type and concentration of different carbon sources, nitrogen sources and inorganic salts, the medium pH, the degree of disintegration of medicinal materials, the volume of liquid medium, the rotating speed of the stirrer, And wogonin were used as evaluation indexes. The optimum conditions for the liquid fermentation of baicalin to produce baicalein and wogonin were peptone 1.0%, sodium chloride 0.05%, p H 6.0 and baicalin 40 mesh, loading volume 33%, shaking speed 200 r · min -1, liquid ratio 5: 1, temperature 37 ℃, fermentation 6 days, baicalin baicalin conversion was 97.6% Baicalin conversion was 97.0%. Validation experiments show that the process is stable and feasible, providing data reference for the industrial production of baicalein and wogonin.