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目的研究辛硫磷对雄性大鼠生殖内分泌系统的影响。方法将每日不同剂量辛硫磷(5.9、29.4、147.0)mg/kg分别对雄性成年SD大鼠连续灌胃染毒15 d和30 d,应用RIA法测定血清卵泡刺激素(FSH)、黄体生成素(LH)、睾酮(T)和睾丸匀浆中睾酮(T)的水平,同步测定睾丸标志酶酸性磷酸酶(ACP)、γ-谷氨酰转移酶(γ-GT)的活性,并采用精子头计数法观测每日精子生成量(Spr)的变化。结果与对照组比较,染毒大鼠15 d时,血清LH水平5.9 mg/kg,各染毒组表现为显著升高(P<0.05);血清FSH的水平随染毒剂量增加而升高,各染毒组均明显高于对照组(P<0.01);血清T水平随染毒剂量的增加呈现为升高的趋势,在147.0 mg/kg剂量组差异有统计学意义(P<0.05)。染毒至30 d时,血清中LH水平在147.0 mg/kg剂量组差异有统计学意义(P<0.05);FSH在≤29.4 mg/kg剂量组表现有统计学意义(P<0.05)。ACP各染毒组有统计学意义(P<0.01)。γ-GT的活性在≥29.4 mg/kg剂量组范围均有明显差异(P<0.01)。Spr与染毒剂量有明显的剂量依赖关系,在≥29.4 mg/kg剂量范围Spr显著减少(P<0.01)。结论辛硫磷对雄性大鼠有明显的生殖毒性,可影响其血清及睾丸性激素水平和酶活性,导致精子生成障碍。
Objective To study the effects of phoxim on reproductive and endocrine system in male rats. Methods Male adult Sprague Dawley rats were administered intragastrically with different doses of phoxim (5.9,29.4,147.0) mg / kg for 15 d and 30 d, respectively. Serum follicle stimulating hormone (FSH), corpus luteum Testosterone (T) and testosterone (T) levels in testis homogenates were measured simultaneously. The activity of testis acid phosphatase (ACP) and γ-glutamyl transferase (γ-GT) Sperm head count was used to observe changes in daily sperm production (Spr). Results Compared with the control group, serum LH level was 5.9 mg / kg on the 15th day, and the levels of serum FSH were increased with the increase of the dose in each exposure group (P <0.05) (P <0.01). The level of serum T increased with the increase of the dose, and the difference was statistically significant at the dose of 147.0 mg / kg (P <0.05). The levels of serum LH in 147.0 mg / kg dose group had significant difference (P <0.05) after exposure to 30 d, and FSH ≤ 29.4 mg / kg dose group had statistical significance (P <0.05). ACP exposure groups were statistically significant (P <0.01). γ-GT activity in the range of ≥29.4 mg / kg dose were significantly different (P <0.01). There was a significant dose-dependent relationship between Spr and the dose of Spr, and the Spr was significantly decreased (P <0.01) at the dose of 29.4 mg / kg. Conclusion Phoxim on male rats has obvious reproductive toxicity, which can affect the serum and testicular sex hormone levels and enzyme activity, resulting in spermatogenesis disorders.