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采用PCR方法克隆大豆光周期GmGBP1基因启动子,构建含有GmGBP1启动子驱动报告基因GUS的融合性表达载体pBI121-pGmGBP1::GUS,通过花序浸泡法,转化野生型拟南芥获得pBI121-pGmGBP1::GUS转基因植株,对T3代转基因拟南芥进行GUS染色,研究了GmGBP1启动子受激素和外源环境诱导的表达规律。结果表明:大豆GmGBP1启动子受水杨酸(SA)和干旱(PEG)以及高温等外源环境的诱导,与生物信息学预测GmGBP1启动子序列存在的逆境胁迫响应元件的结果相一致。
The promoter of GmGBP1 gene was cloned by PCR and the fusion expression vector pBI121-pGmGBP1 :: GUS containing GmGBP1 promoter-driven reporter gene GUS was constructed. The pBI121-pGmGBP1 :: pGmGBP1 :: GUS was transformed into wild type Arabidopsis thaliana by inflorescence, GUS transgenic plants, GUS staining of T3 transgenic Arabidopsis was conducted to study the expression regulation of GmGBP1 promoter induced by hormone and exogenous environment. The results showed that the promoter of soybean GmGBP1 was induced by exogenous environment such as salicylic acid (SA), drought (PEG) and high temperature, and was consistent with the results of bioinformatics prediction of the stress response elements existing in the GmGBP1 promoter sequence.