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adr 基因 Trp64Arg 突变儿童(13名),其体重、BMI 增长幅度明显低于非膳食干预组(分别为2.41±0.56对4.43±0.70,P<0.05;0.48±0.24对1.55±0.35,P<0.05),然而携带有β_3-adr 基因 Trp64Arg 突变儿童(23名)经3个月膳食干预,体重及 BMI 增长幅度与非膳食干预对照组相比无显著性差异(分别为4.32±0.69对4.43±0.70;1.47±0.32对1.55±0.35)。结论:虽然β_3-adr 基因 Trp64Arg 突变可能不是影响汉族儿童单纯性肥胖的主要因素,但该突变参与了肥胖的发病,对肥胖儿童的膳食干预效果可能有一定的预测作用。目的:探讨β_3-肾上腺素能受体(β_3-adr)基因突变与汉族儿童单纯性肥胖、血脂及胰岛素水平的关系;分析β_3-adr 基因突变与肥胖儿童对膳食干预应答差异的关系。方法:在北京城区4所小学中随机选取311名8～11岁健康儿童,其中体重超重肥胖儿童73名,非体重超重儿童243名。所有研究对象均用PCR-RFLP 方法检测β_3-adr 基因中编码多肽链第64位的色氨酸密码子被精氨酸密码子(Trp64Arg)取代突变,并进行身体测量及血脂、胰岛素检测,从体重超重儿童中选出47名单纯性肥胖儿童。将其随机分为两组,第一组36人为膳食干预组,第二组11人为对照组。膳食干预3个月再次进行体格测量。结果:73名体重超重儿童突变频率为0.18,正常儿童突变频率为0.17,两组差异无显著性;在修正了年龄、性别后,311名儿童中有基因突变组与无突变组比较,未发现体质指数(BMI)、血压、血脂及空腹胰岛素水平存在显著性差异;膳食干预研究发现,在膳食干预组无β_3-
Adr gene Trp64Arg mutation in children (13), the body weight, BMI growth was significantly lower than the non-diet intervention group (2.41 ± 0.56 vs 4.43 ± 0.70, P <0.05; 0.48 ± 0.24 vs 1.55 ± 0.35, P <0.05) However, there was no significant difference between the 23 children with Trp64Arg mutation carrying β_3-adr gene and the non-diet intervention group after 3 months of meal intervention (4.32 ± 0.69 vs. 4.43 ± 0.70, respectively; 1.47 ± 0.32 vs. 1.55 ± 0.35). CONCLUSION: Although the mutation of Trp64Arg of β_3-adr gene may not be the major factor affecting simple obesity in Han children, this mutation is involved in the pathogenesis of obesity and may have some predictive effects on the dietary intervention in obese children. OBJECTIVE: To investigate the relationship between β_3-adr gene mutation and simple obesity, blood lipids and insulin levels in Han children and to analyze the relationship between β_3-adr gene mutation and obese children’s response to dietary interventions. Methods: A total of 311 healthy children aged 8 ~ 11 were randomly selected from 4 elementary schools in Beijing urban area, including 73 overweight and obese children and 243 non - overweight children. All of the subjects were detected by PCR-RFLP method β_3-adr gene coding polypeptide chain 64th tryptophan codon by arginine codon (Trp64Arg) substitution mutation, and body measurements and blood lipids, insulin detection from Out of overweight children 47 were selected obese children. They were randomly divided into two groups, the first group of 36 people for the diet intervention group, the second group of 11 people for the control group. Meal intervention 3 months again physical measurements. Results: The frequency of mutation in 73 overweight children was 0.18, and the frequency of mutation in normal children was 0.17. There was no significant difference between the two groups. After adjusting for age and gender, there was no genetic mutation in 311 children Body mass index (BMI), blood pressure, blood lipids and fasting insulin levels were significantly different; diet intervention study found that dietary intervention group without β_3-