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本文采用ＲＴ－ＰＣＲ技术从ＬＰＳ刺激后的小鼠腹腔巨噬细胞，扩增小鼠ＩＬ－６ｃＤＮＡ，并克隆构建ｐＧＥＭ－３Ｚｆ（＋）ＩＬ－６质粒，继将小鼠所得ｃＤＮＡ克隆到ｐＶＬ１３９２载体上，利用杆状病毒ＡｃＮＰＶ表达系统在Ｓｆ９中进行瞬间表达，用ＩＬ－６依赖细胞株ＭＨ６０·ＢＳＦ２检测其表达活性，结果表明，感染后４８ｈ后就具有明显ＩＬ－６表达，由此可见，利用该系统可成功地表达小鼠ＩＬ－６基因。
In this study, murine peritoneal macrophages stimulated by LPS were amplified by RT-PCR from mouse peritoneal macrophages. The mouse IL-6 cDNA was amplified and cloned to construct pGEM-3Zf (+) IL-6 plasmid. The resulting cDNA was cloned into pVL1392 vector The expression of AcNPV was detected in Sf9 cells by transient expression method. The expression of AcNPV was detected in Sf9 cells. The expression of IL-6 was detected by IL-6-dependent cell line MH60 · BSF2. The results showed that IL- The system can be successfully used to express mouse IL-6 gene.