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[Objective] To discuss the effects of resveratrol on the proliferation and cell cycle of human hepatoblastoma-derived HepG-2 cell line. [Methods]The resveratrol was extracted by microwave-assisted method and HepG-2 cells were exposed to resveratrol of different concentrations which were 12. 5,25,50 and 100 μmol /L. The inhibiting effect of resveratrol on the proliferation of HepG-2 cells was measured by MTT. Morphologic changes of cells were detected by the phase contrast microscope. The cell cycle of HepG-2 cells treated with resveratrol were analyzed by flow cytometry. [Results]Resveratrol could significantly inhibit proliferation of HepG-2 cells and the effect was time and concentration dependent. According to results of FCM,resveratrol could block HepG-2 cells into S phase and the effect was dose dependent. [Conclusions]Resveratrol could inhibit the proliferation of HepG-2 cells and induce apoptosis. It could induce cell blockage in S phase.
[Objective] To discuss the effects of resveratrol on the proliferation and cell cycle of human hepatoblastoma-derived HepG-2 cell line. [Methods] The resveratrol was extracted by microwave-assisted method and HepG-2 cells were exposed to resveratrol of different concentrations These were 12. 5, 25, 50 and 100 μmol / L. The inhibiting effect of resveratrol on the proliferation of HepG-2 cells was measured by MTT. Morphologic changes of cells were detected by the phase contrast microscope. The cell cycle of HepG -2 cells treated with resveratrol were analyzed by flow cytometry. [Results] Resveratrol could significantly inhibit proliferation of HepG-2 cells and the effect was time and concentration dependent. According to results of FCM, resveratrol could block HepG-2 cells into S phase and the effect was dose dependent. [Conclusions] Resveratrol could inhibit the proliferation of HepG-2 cells and induce apoptosis. It could induce cell blockage in S phase.