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目的:建立人血浆甲砜霉素检测的高效液相色谱方法。方法:血浆经乙酸乙酯-正己烷萃取,色谱柱:Agilent TC-C_(18);流动相:乙腈-0.1%三氟乙酸-水(20:20:60),流速:0.8 ml·min~(-1);检测波长:225 nm。结果:甲砜霉素浓度在0.10～10.00 mg·L~(-1)范围内线性关系良好(r=0.999 9);高中低三个浓度(0.25,2.50,7.50 mg·L~(-1))的相对回收率分别为(99.20±4.41)%、(98.07±3.94)%和(100.07±1.69)%;日内RSD分别为5.28%、5.19%和1.97%,日间RSD分别为4.38%、3.95%和1.33%。结论:本方法简便、快速、准确可靠,适用于人血浆甲砜霉素浓度的测定及其药代动力学研究。
Objective: To establish a HPLC method for the detection of thiamphenicol in human plasma. Methods: The plasma was extracted with ethyl acetate-n-hexane. The column was Agilent TC-C 18 with a mobile phase of acetonitrile 0.1% trifluoroacetic acid and water (20:20:60) (-1); detection wavelength: 225 nm. Results: Thiamphenicol had good linearity in the range of 0.10-10.00 mg · L -1 (r = 0.999 9), high, medium and low concentrations (0.25, 2.50 and 7.50 mg · L -1) ) Were (99.20 ± 4.41)%, (98.07 ± 3.94)% and (100.07 ± 1.69)%, respectively. The intraday RSD was 5.28%, 5.19% and 1.97% respectively. The daytime RSD was 4.38% and 3.95 respectively % And 1.33%. Conclusion: The method is simple, rapid, accurate and reliable, suitable for the determination of plasma thiamphenicol concentration and its pharmacokinetics.