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为了进一步探讨癌基因表达的变化与辐射诱导肿瘤细胞凋亡的关系，作者采用免疫组化技术，检测了人脑星形细胞瘤细胞系经过Ｘ射线处理前后ｐ５３、ｃ－ｍｙｃ和ｂｃｌ－２基因表达的变化；采用原位杂交方法检测ｐ５３基因在ｍＲＮＡ水平表达的变化。结果表明：①凋亡组内ｐ５３和ｃ－ｍｙｃ基因表达率明显高于对照组（Ｐ＜０．０１和Ｐ＜０．０５），ｂｃｌ－２表达显著减弱（Ｐ＜０．０５），ｐ５３或ｃ－ｍｙｃ与Ｂｃｌ－２基因表达呈显著性负相关（Ｐ＜０．０５）。②凋亡组内ｐ５３在ｍＲＮＡ水平表达量明显高于对照组（Ｐ＜０．０５），ｐ５３基因在蛋白水平与ｍＲＮＡ水平表达呈显著性正相关（Ｐ＜０．０５）。③ｐ５３和ｃ－ｍｙｃ基因对细胞凋亡具有增殖和凋亡双向调节作用，ｂｃｌ－２基因抑制细胞凋亡，三者在细胞凋亡过程中存在着协同或拮抗作用的关系。本研究结果为脑胶质瘤的基因治疗提供了一条新线索。
In order to further explore the relationship between the changes of oncogene expression and radiation-induced apoptosis of tumor cells, the authors used immunohistochemistry to detect the expression of p53, c-myc and bcl-2 genes in human astrocytoma cell lines before and after X-ray treatment Expression changes; using in situ hybridization detection of p53 gene expression at the mRNA level changes. The results showed that: (1) The expression of p53 and c-myc in the apoptosis group was significantly higher than that in the control group (P <0.01 and P <0.05), the expression of bcl-2 was significantly decreased Or c-myc and Bcl-2 gene expression was significantly negatively correlated (P <0.05). ② The expression of p53 in the apoptosis group was significantly higher than that in the control group (P <0.05). There was a significant positive correlation between the expression of p53 protein and mRNA level (P <0.05). ③p53 and c-myc gene have a two-way regulation of apoptosis on cell apoptosis, bcl-2 gene inhibits cell apoptosis, and the three have a synergistic or antagonistic effect on cell apoptosis. The results of this study provide a new clue for the gene therapy of glioma.