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目的探讨整片组织内淋巴管免疫染色,并与间接注射法和组织切片免疫染色法比较。方法用普鲁士蓝间接注射新生儿肝被膜内淋巴管,观察肝浅淋巴管的构筑。通过火棉胶切片分析肝浅淋巴管的流向。取人大腿皮肤,作石蜡切片和冷冻切片,免疫染色后观察淋巴管的分布。取大鼠耳和背部的皮肤、膈和小肠,将整片组织作免疫染色,观察组织内淋巴管构筑。结果间接注射普鲁士蓝可显示淋巴管构筑和流向,但淋巴管染色缺乏特异性。组织切片免疫染色可显示淋巴管分布,然而不能准确地分析淋巴管密度。整片组织的免疫染色很理想,毛细淋巴管盲端、毛细淋巴管网和淋巴管丛清晰可见。结论整片组织免疫染色能够很好地显示组织内完整的淋巴管构筑。
Objective To investigate the immunostaining of lymphatic vessels in the whole tissue and compare with indirect immunostaining and histological section immunostaining. Methods Indirect injection of Prussian Blue into the lymphatic capillaries of the liver of newborn to observe the construction of lymphatic vessels of the liver. Analysis of the direction of the lymphatic vessels in the liver by collodione section. Take thigh skin for paraffin sections and frozen sections, lymphatic distribution observed after immunostaining. Take the ears and back of the rat skin, diaphragm and small intestine, the entire tissue for immunostaining, observe the construction of lymphatic vessels within the organization. Results Indirect injection of Prussian blue showed lymphangiogenesis and flow, but lack of specificity for lymphatic staining. Tissue sections immunostaining can show lymphatic vessel distribution, however, lymphatic vessel density can not be accurately analyzed. Immunostaining of the whole tissue is ideal, with clear ends of the lymphatic capillaries, lymphatic vessels and lymphatic vessels clearly visible. Conclusion The whole tissue immunostaining can well show the complete lymphatic vessel construction in the tissue.