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AIM:To study the mechanism of cerebral pathological and pathophysiological cha nges after extremity gunshot wounds at molecular level.METHODS:M-193,5.56 mm bu llets were used to shoot the most plentiful part of the hind extremities of the dogs.C-fos gene expression in neurons of cerebral tissues of different time int ervals after gunshot in the subject and control groups were detected with immuno histochemistry.RESULTS:No C-fos gene expression was detected in the cerebral co rtical neurons in the control group.But in the subject group,C-fos expression i ncreased in the cerebral neurons 30 min after injury,reached the peak 6 hours(P< 0.05),and began to decrease 10 hours post-injury.CONCLUSION:After extremity gu nshot wound C-fos expression in neurons of distal parts is the early response o f the body to the injury,which might be caused by Leao’s spreading depression(SD ),and correlated to extracellular transduction and cell apoptosis.
AIM: To study the mechanism of cerebral pathological and pathophysiological changes after extremity gunshot wounds at molecular level. METHODS: M-193, 5.56 mm bu llets were used to shoot the most plentiful part of the hind extremities of the dogs.C-fos gene expression in neurons of cerebral tissues of different time int ervals after gunshot in the subject and control groups were detected with immunohistochemistry .RESULTS: No C-fos gene expression was detected in the cerebral co rtical neurons in the control group.But in the subject group, C-fos expression i ncreased in the cerebral neurons 30 min after injury, reached the peak 6 hours (P <0.05), and began to decrease 10 hours post-injury. CONCLUSION: After extremity gu shots C-fos expression in neurons of distal parts is the early response of the body to the injury, which might be caused by Leao’s spreading depression (SD), and correlated to extracellular transduction and cell apoptosis.