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用种子萌发获得颠茄无菌苗,用发根农杆菌A4侵染颠茄无菌苗真叶,所有感染了的叶片都从伤口处产生发根．在无激素培养基中,发根表现出高频侧向分支、生长迅速、失去向地性的典型形态特征．PCR检测表明rolB、rolC确实整合到颠茄发根基因组中,用MS液体培养基发酵培养35 d,3个发根单克隆中发根生物量最大增长率达7．5倍,T6单克隆莨菪碱含量最高(5．61 mg／g),与种植在田间的颠茄植株的根相比,提高7倍;T2单克隆东莨菪碱含量最高,提高9倍多(2．35 mg／ g)．本研究揭示了rol基因足以诱导获得生长迅速和次生代谢产物积累的发根．表明颠茄转基因发根的发酵培养不失为生产托品烷类生物碱的一条好途径．
Obtained with seed germination Pieris aseptic seedlings, Agrobacterium rhizogenes A4 infection of true leaves of Belladonna aseptic seedlings, all infected leaves produce hair roots from the wound. In hormone-free medium, the hairy roots showed high-frequency lateral branches, growing rapidly and losing the typical morphological characteristics of geotropism. PCR assay showed that rolB and rolC were indeed integrated into the hairy root genome of Belladonna tuber and cultured in liquid MS medium for 35 days. The maximum growth rate of hairy root biomass in three hairy root clones reached 7.5 times. The highest alkali content (5.61 mg / g) was 7-fold higher than that of the plants grown in the field. The content of scopolamine in T2 was the highest (2.35 mg / g). The present study revealed that the rol gene is sufficient to induce hair root growth with rapid growth and secondary metabolite accumulation. That the transformation of Arabidopsis hairy root fermentation is a good way to produce alkaloids alkaloids.