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目的 检测喉癌组织人类主要组织相容性抗原 (humanleucotyteantigen ,HLA)表达缺失情况 ,探讨其表达与肿瘤浸润淋巴细胞 (tumorinfiltratinglymphocyte ,TIL)及肿瘤细胞恶性度的关系。方法 2 0 0 1年 2月～ 2 0 0 1年 5月应用特异性鼠抗人HLAⅠ类分子等单克隆抗体与喉癌组织结合 ,通过免疫组化法 ,对 1993～ 1998年 6 4例手术切除喉癌标本进行检测。结果 6 4例喉癌组织中有 5 7例HLAⅠ抗原阳性表达 ,7例阴性表达 ,阴性表达率为 10 9% (7/ 6 4 ) ;HLAⅠ抗原阳性组织 ,浸润到肿瘤实质的CD+ 3 T细胞和CD+ 8T细胞数量明显多于HLAⅠ抗原阴性组织 ;随着HLAⅠ抗原表达下调 ,肿瘤细胞恶性度增加。随访 5 2例 ,3年生存率与HLAⅠ抗原缺失无关 (P >0 0 5 )。结论 部分喉癌组织存在HLAⅠ抗原丢失 ,且其丢失不利于细胞毒性T淋巴细胞向肿瘤实质浸润与杀伤。HLAⅠ抗原表达缺如 ,可能促进了细胞恶变的发生发展 ,是肿瘤逃避免疫监视的方式之一
Objective To detect the loss of human major histocompatibility antigen (HLA) expression in laryngeal carcinoma and to investigate its relationship with tumor infiltrating lymphocyte (TIL) and tumor cell malignancy. Methods From February 2001 to May 2001, monoclonal antibodies against human HLA class I and other monoclonal antibodies were combined with laryngeal cancer tissues. Immunohistochemical staining was performed on 64 cases of surgical operations from 1993 to 1998 Excision of laryngeal specimens for testing. Results The positive rate of HLA Ⅰ antigen was 57 in 64 cases of laryngeal squamous cell carcinoma, 7 cases were negative, and the negative rate was 10 9% (7/4 4). HLA Ⅰ antigen-positive tissues infiltrated into the tumor-bearing CD + 3 T cells And the number of CD + 8T cells was significantly higher than that of HLA I antigen negative cells. With the down-regulation of HLA I expression, the malignant degree of tumor cells increased. Follow-up 52 cases, 3-year survival rate has nothing to do with HLA I antigen deletion (P> 0.05). Conclusion Some HLA-I antigen is lost in some laryngeal cancer tissues, and its loss is not conducive to the infiltration and killing of cytotoxic T lymphocytes to the tumor. The lack of expression of HLA I antigen may promote the occurrence and development of malignant cells, which is one of the ways of tumor escape immune surveillance