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目的:探讨适合我们县级疾控开展群体性流感样发热疫情调查处理的病原学检测方法。方法:采用ELISA法和间接免疫荧光法,对流感样病例的血清样品进行甲/乙型流感病毒及其它病原体的IgM抗体检测;采用胶体金免疫色谱法,对流感样病例的咽拭子进行甲/乙型流感病毒抗原检测。结果:ELISA法检测11起疫情中62份血清样品的甲/乙型流感病毒IgM抗体,两者同时阳性38.71%(24/62);间接免疫荧光法检测13起疫情中65份血清样品的甲/乙型流感病毒IgM抗体,两者同时阳性46.15%(26/65+4/65);胶体金免疫色谱法检测44起疫情173份咽拭子的甲/乙型流感病毒抗原,甲型22.54%(39/173),乙型32.95%(57/173),两者无同时阳性。结论:我们经近六年的实际应用体会到,流感胶体金免疫色谱法明显优于ELISA法和间接免疫荧光法,适用于我们尚无条件开展病毒细胞培养和病毒核酸检测的基层疾控。
OBJECTIVE: To explore the etiological detection method suitable for investigation and treatment of swine flu-like fever in our county-level disease control. Methods: Serum samples of influenza-like cases were tested for IgM antibody against influenza A / B and other pathogens by ELISA and indirect immunofluorescence. Thyroid swabs were treated with colloidal gold immunochromatography / Influenza B virus antigen test. Results: The results of ELISA showed that 62 serogroup A / B influenza IgM antibodies were detected in 11 of the 11 outbreaks, both of which were 38.71% (24/62) positive at the same time. Indirect immunofluorescence assay detected 63 serum samples from 13 outbreaks / Influenza B virus IgM antibody, both simultaneously positive 46.15% (26/65 + 4/65); colloidal gold immunochromatographic assay of 44 outbreaks of 173 throat swabs of influenza A / B antigen, A 22.54 % (39/173), type B 32.95% (57/173), both positive and negative. Conclusion: After nearly six years of practical application, we have realized that influenza colloidal gold immunochromatography is obviously better than ELISA and indirect immunofluorescence, which is suitable for the grass-roots disease that we do not yet carry out virus cell culture and virus nucleic acid detection.