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Exclusion and imflammation in the clinic are observed for various reasons including material chemical composition, physical properties as well as macro- and micro-structure of the implants, surface condition of the implants, and also patient dependent factors. Cytotoxicity expression of cells is a central issue in current biocompatibility to screen the potential implant materials and drugs. This study was aimed at investigation reaction between the potential implant materials and surround tissue. Cytotoxicity of human and mt osteoblast in the material extracts was determinated by testing standards such as GHS assay, MTI assay, alkaline phosphatase activity assay, LDH assay, and Lowry assay. Research results demonstrated that compared with the control condition polystyrene culture plate both human and rat osteoblast cells have normal phenotypie expression in hydroxyapatite extract, and this expression was statistically restricted in hydroxyapatite-spinel extract. However, this restrict, e.g. cytotoxicity could be partially eliminated by immersion treatment of the materials in culture medium.