论文部分内容阅读
目的探讨发育期大鼠癫痫持续状态对其海马内低氧诱导因子-1α(HIF-1α)和血红素加氧酶-1(HO-1)表达的影响。方法 21 d SD大鼠随机分为2组:生理盐水组(NS组)、戊四氮(PTZ)致癫痫持续状态模型组(模型组)。1%PTZ(80 mg/kg)予大鼠腹腔注射诱导建立癫痫持续状态模型,生理盐水组予等量生理盐水腹腔注射,采用免疫组化和Western blot分别检测海马内HIF-1α、HO-1蛋白和阳性细胞数的表达。结果 HIF-1α、HO-1蛋白在正常生理状态下(NS组)均表达,而且表达平稳;在模型组存在明显的表达时程和表达高峰变化,HIF-1α蛋白1 h即有表达,4 h增多,8 h达高峰,然后逐渐下降;HO-1蛋白1 h少量表达,4~8 h逐渐增多,12 h达高峰,后渐下降。结论癫痫持续状态后,HIF-1α和HO-1表达升高可能是癫痫后的一个重要的脑保护机制。
Objective To investigate the effects of status epilepticus on the expression of hypoxia inducible factor-1α (HIF-1α) and heme oxygenase-1 (HO-1) in developing rat hippocampus. Methods Twenty-two SD rats were randomly divided into two groups: normal saline group (NS group) and pentylenetetrazole (PTZ) -contained epilepsy model group (model group). The rats were injected intraperitoneally with 1% PTZ (80 mg / kg) to induce the status epilepticus. The rats in the saline group were injected intraperitoneally with the same volume of saline. The expressions of HIF-1α and HO-1 were detected by immunohistochemistry and Western blot Expression of protein and positive cells. Results The expression of HIF-1α and HO-1 protein in normal physiological condition (NS group) were both stable and stable. The expression of HIF-1α in the model group was significantly increased at 1 h h increased, peaked at 8 h, and then decreased gradually. HO-1 protein was little expressed at 1 h, gradually increased at 4 ~ 8 h, peaked at 12 h, and then decreased gradually. Conclusion After the status epilepticus, the increased expression of HIF-1α and HO-1 may be an important brain protection mechanism after epilepsy.