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目的:研究新藤黄酸对宫颈癌HeLa细胞增殖、凋亡、迁移的影响及其机制。方法:不同浓度新藤黄酸(0.00、0.63、1.25、2.50、5.00、10.00 mg/L)作用于HeLa细胞72 h,MTT法检测细胞增殖;流式细胞术检测细胞周期;荧光显微镜和DNA ladder检测细胞凋亡;Transwell实验检测细胞迁移;RT-PCR、Western blot检测BCL-2、BAX、NF-κB及E-cadherin的mRNA和蛋白表达。结果:不同浓度新藤黄酸作用HeLa细胞72 h后,细胞抑制率随药物浓度升高而升高;穿过Transwell小室的细胞数随药物浓度升高而减少;BCL-2、NF-κB mRNA及蛋白表达随新藤黄酸浓度升高而降低;BAX、E-cadherin mRNA及蛋白表达随新藤黄酸浓度的升高而升高。结论:新藤黄酸在一定范围内能以剂浓度依赖性抑制HeLa细胞增殖,促进其凋亡,并抑制其迁移。
Objective: To study the effects of new gambogic acid on proliferation, apoptosis and migration of cervical cancer HeLa cells and its mechanism. Methods: HeLa cells were treated with different concentrations of new gambogic acid (0.00,0.63,1.25,2.50,5.00,10.00 mg / L) for 72 h. Cell proliferation was detected by MTT assay. Cell cycle was detected by flow cytometry. Fluorescence microscopy and DNA ladder Cell apoptosis was detected by Transwell assay. The mRNA and protein expression of BCL-2, BAX, NF-κB and E-cadherin were detected by RT-PCR and Western blot. Results: After 72 h treated with different concentrations of Gambogic acid, the cell inhibitory rate increased with the increase of drug concentration. The number of cells passing through Transwell chamber decreased with the increase of drug concentration. The expressions of BCL-2, NF-κB mRNA, The expression of BAX and E-cadherin mRNA and protein were increased with the concentration of neo gambogic acid. Conclusion: Gambogic acid can inhibit HeLa cell proliferation, promote apoptosis and inhibit its migration in a concentration-dependent manner.