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目的:研究骨显像剂n 99Tcn m-亚甲基二膦酸盐(MDP)在骨损伤修复不同阶段的分布状况。n 方法:用电钻股骨凿孔的方法，制作兔股骨损伤模型共30只，根据骨损伤修复的不同阶段(第1、2和3周)分成A、B和C组，每组10只。在骨损伤修复不同阶段的最后1 d行股骨SPECT/CT显像，得到实验侧和对照侧感兴趣区(ROI)放射性计数，计算靶/本底比值(T/B)。应用磷屏成像分析3组模型标本光强度，放射自显影观察n 99Tcn m-MDP在骨细胞的具体分布情况。采用单因素方差分析和配对n t检验分析数据。n 结果:SPECT/CT显像示A、B和C组的放射性计数T/B分别为1.16±0.14、1.39±0.23和1.18±0.10，差异有统计学意义(n F＝5.83，n P<0.01)。A、B和C组实验侧(50.00±12.45、59.50±12.83和55.10±9.26)的最大放射性计数均高于对照侧(43.20±9.57、50.00±12.30和44.30±6.50；n t值：3.24，2.28和5.77，均n P<0.05)。磷屏成像示A、B和C组股骨标本的光强度差异有统计学意义(37 324.67±6 481.50、60 950.33±9 781.72和43 905.00±4 957.92；n F＝8.25，n P＝0.02)。放射自显影观察到在骨修复的不同阶段，n 99Tcn m-MDP在骨细胞和成骨细胞的细胞内和细胞外均有分布。n 结论:在骨损伤修复的不同阶段，n 99Tcn m-MDP均有显著的分布浓聚，提示其在骨组织中浓聚机制除与羟基磷灰石的化学吸附作用外，还存在其他途径。n “,”Objective:To investigate the distribution of n 99Tcn m-methylene diphosphonate (MDP) at different stages of bone injury repair.n Methods:A total of 30 rabbit models of femur injury were established by the method of electric drill and perforation of femur. According to the different stages of bone injury repair (at 1, 2 and 3 week), rabbits were divided into group A, B and C (n n=10 each group). Femoral SPECT/CT imaging was performed on the last day of different stages of bone injury repair to obtain radioactivity counts in the region of interest (ROI) on the test side and control side and to calculate target/background ratio (T/B). The light intensity of 3 groups was analyzed by phosphor screen imaging and the distribution of n 99Tcn m-MDP in bone cells was observed by autoradiography. One-way analysis of variance and paired n t test were used to analyze the data.n Results:The T/B values of group A, B and C were 1.16±0.14, 1.39±0.23 and 1.18±0.10, respectively (n F=5.83, n P<0.01). There were significant differences of the maximum radiation count between the test side (50.00±12.45, 59.50±12.83 and 55.10±9.26) and the control side (43.20±9.57, 50.00±12.30 and 44.30± 6.50) in group A, B and C (n t values: 3.24, 2.28 and 5.77, all n P<0.05). There were significant differences in the light intensity of bone specimens in group A, B and C by phosphor screen imaging (37 324.67±6 481.50, 60 950.33±9 781.72 and 43 905.00±4 957.92;n F=8.25, n P=0.02). n 99Tcn m-MDP were deposited in both intracellular and extracellular during different stages of bone repair in osteocytes and osteoblasts under autoradiography.n Conclusion:At different stages of bone injury repair, the concentration of n 99Tcn m-MDP is significantly distributed, suggesting that there are other ways of concentration mechanism of n 99Tcn m-MDP in bone tissue besides the chemical adsorption with hydroxyapatite.n