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This study aimed to survey the expression of genes involved in rice N uptake and aasimilatory network and to understand the potential molecular mechanisms responsible for the NO3-enhanced NH+ uptake.By using quantitative real-time polymerase chain reaction (PCR),the genes related to N nutrition,including ammonium transporters (AMTs) and ammonium assimilatory enzymes (GS and GOGAT),were transcriptionally analyzed in rice plants grown in the absence and presence of NO_3~- in the NH_4~+-containing medium.The results showed that NH_4~+ uptake by rice was enhanced by the NO_~3 supply to the medium.At the same time and in parallel,the amount of transcripts of seven genes (OsAMT1;1, OsAMT1;2,OsAMT4;1,OsGLN2,OsGLU1,OsGLT1,and OsGLT2) was increased in rice roots,but the expression of two genes (OsGLN1;1 and OsGLN1;2) was decreased and that of OsAMT1;3 remained without change.Up-or down- regulation of these genes involved in NH_~+ uptake and assimilation correlated with the increase in NH_~+ uptake in the presence of NO_3~- in rice roots.
This study aimed to survey the expression of genes involved in rice N uptake and aasimilatory network and to understand the potential molecular mechanisms mechanisms for the NO3-enhanced NH + uptake. BY using quantitative real-time polymerase chain reaction (PCR) N nutrition, including ammonium transporters (AMTs) and ammonium assimilatory enzymes (GS and GOGAT), were transcriptionally analyzed in rice plants grown in the absence and presence of NO_3 ~ - in the NH_4 ~ + -containing medium. The results showed that NH_4 ~ + uptake by rice was enhanced by the NO_ ~ 3 supply to the medium. At the same time and in parallel, the amount of transcripts of seven genes (OsAMT1; 1, OsAMT1; 2, OsAMT4; 1, OsGLN2, OsGLU1, OsGLT1, and OsGLT2) was decreased in rice roots, but the expression of two genes (OsGLN1; 1 and OsGLN1; 2) was decreased and that of OsAMT1; 3 remained without change. Up- or down- regulation of these genes involved in NH_ ~ uptake and assimilation correlated with the increase in NH_ ~ + upt ake in the presence of NO_3 ~ - in rice roots.