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[目的]探讨转化生长因子(TGF)-β和肿瘤坏死因子(TNF)-α基因多态性在矽肺发病中的作用。[方法]选择259名汉族男性矽肺患者为病例组,341名矽尘接触者为对照组,填写统一的调查问卷、拍摄高仟伏X射线后前位胸片、采集外周静脉血,采用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测TGF-β(+869)位点和TNF-α(-238)位点基因多态性。[结果]病例组TGF-β(+869)位点和TNF-α(-238)位点等位基因分布频率与对照组比较,差异均无统计学意义。将病例和对照组按工种、工龄、年龄进行1:1匹配后,TNF-α(-238)位点GA基因型发生矽肺的危险性明显降低(OR=0.27,95%CI:0.10～0.75,P<0.05)。[结论]TGF-β(+869)位点的多态性与矽肺的易感性无关,TNF-α(-238)位点的多态性可能减少患矽肺的危险性。
[Objective] To investigate the role of transforming growth factor (TGF) -β and tumor necrosis factor-α (TNF-α) gene polymorphisms in silicosis. [Methods] A total of 259 Han patients with silicosis were selected as the case group and 341 as the control group. A questionnaire was filled in. A series of chest radiographs were taken after the X-ray was irradiated. The peripheral blood was collected by polymerase chain reaction The gene polymorphisms of TGF-β (+869) and TNF-α (-238) were detected by PCR-RFLP. [Results] There were no significant differences in allele frequency between TGF-β (+869) and TNF-α (-238) loci in the case group compared with the control group. The risk of silicosis of GA genotype at TNF-α (-238) locus was significantly lower (OR = 0.27, 95% CI: 0.10-0.75, P < P <0.05). [Conclusion] The polymorphism of TGF-β (+869) site is not related to the susceptibility to silicosis. The polymorphism of TNF-α (-238) site may reduce the risk of silicosis.