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目的研究溴隐亭(BRC)对植物血凝素(PHA)诱导活化T淋巴细胞的影响。方法用泌乳素(PRL)与BRC单独和联合刺激培养PHA诱导活化的CD4+T淋巴细胞系JurkatE6-1细胞,利用实时聚合酶链反应(RT-PCR)技术检测活化T淋巴细胞重要信号分子转接蛋白(LAT)、T细胞受体相关的相对分子质量为70000的蛋白激酶ζ链(ZAP-70)mRNA表达变化情况;利用Realtime-PCR方法检测活化T淋巴细胞自身PRLmRNA表达变化情况;利用流式细胞术检测T淋巴细胞活化早期表面标志细胞分化抗原CD25分子表达变化情况;利用脂质体转染荧光素酶报告系统检测T淋巴细胞活化的核转录因子-κB(NF-κB)活性变化情况。结果1.BRC可抑制PHA诱导的T淋巴细胞活化和PRL-泌乳素受体(PRLR)信号转导途径的共同信号分子ZAP-70mRNA及活化T淋巴细胞自身PRLmRNA的表达;2.BRC促进了活化T淋巴细胞信号分子LATmRNA和活化T淋巴细胞表面CD25分子的表达;3.BRC抑制了PHA诱导的活化T淋巴细胞核NF-κB活性。结论BRC可通过抑制T淋巴细胞活化和PRL-PRLR途径中的共同信号分子ZAP-70及活化T淋巴细胞自身PRLmRNA分泌,对T淋巴细胞活化发挥抑制作用。
Objective To investigate the effects of bromocriptine (BRC) on phytohemagglutinin (PHA) -induced activation of T lymphocytes. Methods Jurkat E6-1 cells, activated by PHA, were stimulated with prolactin (PRL) and BRC alone and in combination with BRC. Real-time polymerase chain reaction (RT-PCR) was used to detect the expression of important signaling molecules in activated T lymphocytes LAT, ZAP-70 mRNA expression of T-cell receptor-related relative molecular mass of 70,000; Real-time PCR was used to detect the expression of PRL mRNA in activated T lymphocytes; The changes of expression of CD25 on early surface markers of T lymphocyte activation were detected by cytometry. The changes of activation of nuclear transcription factor-κB (NF-κB) in T lymphocytes were detected by lipofection luciferase reporter system . BRC can inhibit PHA-induced T lymphocyte activation and PRL-PRLR signal transduction pathway common signal molecule ZAP-70mRNA and activation of T lymphocytes PRLmRNA expression; 2.BRC promote the activation T lymphocyte signaling molecule LATmRNA and activated T lymphocyte surface CD25 molecule expression; 3.BRC PHA-induced activation of activated T lymphocyte nuclear NF-κB activity. Conclusion BRC can inhibit the activation of T lymphocytes by inhibiting the activation of T lymphocytes and the common signal molecule ZAP-70 in the PRL-PRLR pathway and the secretion of PRL mRNA by activated T lymphocytes.