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目的:建立微流控芯片非水毛细管电泳激光诱导荧光分离检测博落回种子中白屈菜红碱和血根碱的方法。方法:用简单玻璃十字芯片,以200 mmol·L-1乙酸铵-乙酸-甲酰胺-水(2∶0.5∶5∶2.5,p H=2.90)缓冲溶液作电泳缓冲液,检测距离4 cm,分离电压1.8 k V。结果:在150 s内即可完成白屈菜红碱和血根碱的进样和基线分离。白屈菜红碱质量浓度在0.15~550μg·m L-1范围内呈良好线性(r=0.9993),检测限为5.0 ng·m L-1,平均回收率(n=6)为99.6%;血根碱质量浓度在0.3~600μg·m L-1范围内呈良好线性(r=0.9998),检测限为2.0 ng·m L-1,平均回收率(n=6)为99.2%。结论:本方法快速、准确,样品用量少,可用于分离并定量药材中的白屈菜红碱和血根碱,并为分离结构相似的组分提供了方法借鉴。
OBJECTIVE: To establish a method for the determination of chelerythrine and sanguarinarine in seeds by microfluidic chip non-aqueous capillary electrophoresis with laser-induced fluorescence separation. Methods: A simple glass cross was used as the electrophoresis buffer with 200 mmol·L-1 ammonium acetate-acetic acid-formamide-water (2:0.5:5:2.5, p H = 2.90) Divide the voltage by 1.8 kV. Results: Injection and baseline separation of chelerythrine and sanguinarine were achieved within 150 s. The concentration of chelerythrine was linear in the range of 0.15-550 μg · mL-1 (r = 0.9993), with the detection limit of 5.0 ng · m L-1 and the average recovery (n = 6) of 99.6%. The calibration curve of sanguarinar was linear in the range of 0.3-600μg · m L -1 (r = 0.9998). The detection limit was 2.0 ng · m L-1. The average recoveries (n = 6) were 99.2%. Conclusion: The method is rapid, accurate and can be used for the separation and quantification of chelerythrine and sanguinarine in medicinal materials. It provides a reference for the separation of structurally similar components.