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目的:研究胃癌组织中Smad3基因与Smad7基因的表达水平以及二者的相关性,并探索其临床意义。方法:采用免疫组织化学和RT-PCR的方法,检测50例胃癌,癌旁组织以及10例正常胃壁组织中,Smad3与Smad7,蛋白与mRNA的表达。结果:胃癌组织中Smad3蛋白表达阳性率明显低于癌旁组织(50.0%vs.92.0%,P=0.000),2组相比有统计学差异。并与肿瘤的分期(P=0.004)及肿瘤组织的分化(P=0.030)密切相关。而与年龄(χ2=0.439,P=0.508)、性别(χ2=0.117,P=0.733)均无关,胃癌组织中Smad7蛋白的表达阳性率高于癌旁组织(48.0%vs.4.0%,P=0.000),正常组织最低。且与肿瘤的分期(P=0.009),组织的分化程度(P=0.001)密切相关。在胃癌组织中发现Smad3蛋白与Smad7蛋白呈负相关性(r=-0.539,P=0.000)。Smad3 mRNA在癌组织中的表达低于癌旁组织(Z=7.84,P=0.000),而Smad7 mRNA在癌组织中的表达高于癌旁组织(Z=7.83,P=0.000)。结论:Smad3基因在癌组织中低表达,而Smad7基因在癌组织中则高表达,并且二者与胃癌的分期,组织分化程度有关,提示二者在胃癌的发生、发展中有一定的作用,可能和胃癌的分化,分期有关。
OBJECTIVE: To study the expression of Smad3 and Smad7 in gastric cancer and their correlations, and to explore their clinical significance. Methods: Immunohistochemistry and RT-PCR were used to detect the expression of Smad3, Smad7, protein and mRNA in 50 cases of gastric cancer, paracancerous tissues and 10 cases of normal gastric tissues. Results: The positive rate of Smad3 protein expression in gastric cancer tissues was significantly lower than that in paracancer tissues (50.0% vs.92.0%, P = 0.000). There was a significant difference between the two groups. Which was closely related to tumor stage (P = 0.004) and tumor tissue differentiation (P = 0.030). The positive rate of Smad7 protein expression in gastric cancer tissues was significantly higher than that in adjacent tissues (48.0% vs.4.0%, P = 0.398, P = 0.508), sex (χ2 = 0.117, 0.000), the lowest normal tissue. It was closely related to tumor stage (P = 0.009) and histological differentiation (P = 0.001). Smad3 protein was negatively correlated with Smad7 protein in gastric cancer (r = -0.539, P = 0.000). The expression of Smad3 mRNA in cancerous tissue was lower than that in paracancerous tissue (Z = 7.84, P = 0.000). The expression of Smad3 mRNA in cancerous tissue was higher than that in paracancerous tissue (Z = 7.83, P = 0.000). Conclusion: Smad3 gene is low expressed in cancer tissues, while Smad7 gene is highly expressed in cancer tissues, and both of them are correlated with the stage of gastric cancer and the degree of tissue differentiation, suggesting that both of them play a role in the occurrence and development of gastric cancer. May be related to the differentiation and staging of gastric cancer.