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越来越多的研究表明相关的植物种之间在基因序列和基因顺序上时常保持一定的同源性。之前,我们用110个TCs在重组自交系上构建了覆盖整个大豆染色体组的遗传基因图谱。为了调查大豆与百脉根以及模式植物拟南芥之间同源性的程度,通过Blastn,我们利用这些被定位的TC在GenBank中收集的百脉根和拟南芥序列中收寻类似的基因。结果显示在大豆被定位的110个TC中,39个与百脉根的基因有显著的相似性,并且发现了8个较小的同源区。虽然大豆与拟南芥分化地比较早,至少在九千万年前,仍能找到一些相似的基因和同源区。大豆基因在拟南芥上往往会有1个以上的相似基因,这说明拟南芥在进化过程中经历了大范围的短片复制。大豆与百脉根及拟南芥间的同源区都比较小,这可能是因为大豆的基因组复制以及随后的基因丢失和短片重组造成在大豆与其它植物间很难找到较长的同源区。
More and more researches show that the related plant species always maintain some homology in gene sequence and gene order. Previously, we used 110 TCs to construct a genetic map that covers the whole soybean genome in a recombinant inbred line. In order to investigate the degree of homology between soybean and Lotus japonicus and the model plant Arabidopsis, we used these located TCs to find similar genes in the Lotus japonicus and Arabidopsis sequences collected in GenBank by Blastn . The results showed that among the 110 TCs in which soybean was located, 39 were significantly similar to the genes of Lotus japonica, and 8 smaller homologous regions were found. Although soybean and Arabidopsis differentiated earlier, at least 90 million years ago, some similar genes and regions of homology were still found. Soybean genes often have more than one similar gene on Arabidopsis thaliana, indicating that Arabidopsis has undergone a wide range of short film replication during evolution. Soybean and Lotus japonicus and Arabidopsis homologous regions are relatively small, which may be due to soybean genome duplication and subsequent gene loss and short recombination caused by the soybean and other plants is difficult to find longer homologous regions .