Detecting β-thalassaemia mutations from a single cell by PEP and RDB

• 作 者: 易萍 李力 姚宏 周元国 邓兵 陈竹钦
• 机 构: DepartmentofObstetricsandGynecology,DepartmentofMolecularBiology,Children＇sHospital
• 出 处: 中国人民解放军军医大学学报：英文版
• 发表日期: 2006年3期
• 关键词: Β-地中海贫血 寡核苷酸 基因突变 病理机制 β-thalassaemia preamplification reverse dot blot

<正>Objective :To evaluate the possibility of the technology involving PEP and RDB for detectingβ-thalassaemia multipoint mutations from a single cell simultaneously. Methods: A set of allele specific oligonucleotide (ASO) probes used for detecting 8