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目的:研究舌根隧道法射频治疗中舌根不同部位损伤体积,探讨其治疗的安全性。方法:18个新鲜离体猪舌随机分成6个组,每组3只。参照Powell等介绍方法在猪舌根选取6个射频操作点。用低温等离子射频发生仪进行猪舌根射频操作,取能级6,作用时间10s。作用后的舌根组织块进行连续冷冻切片,间隔200μm保留切片1张,苏木精-伊红染色后,进行序列组织切片的全貌二维图像采集,对损伤区域进行图像分割,提取。将提取分割图像导入IPS图像处理软件,进行损伤区域的三维重建及体积计算。结果:各作用点体积分别为(359.5±5.6)mm3、(364.3±7.0)mm3、(363.7±7.2)mm3、(354.1±11.8)mm3、(349.4±17.2)mm3、(353.5±7.9)mm3,舌根各作用点射频损伤体积差异无统计学意义(P>0.05)。结论:在舌根隧道法射频治疗中,相同作用能级和作用时间下不同作用点损伤体积一致。低温等离子射频治疗系统作用能量集中,舌根区域损伤范围局限,在舌根区域操作具有较高的安全性。
OBJECTIVE: To study the lesion volume in different parts of tongue base in radiofrequency treatment of tongue base tunnel and to explore the safety of its treatment. Methods: Eighteen freshly isolated piglets were randomly divided into six groups of three. Refer to Powell’s introduction method to select 6 RF operating points in pig tongue root. Low temperature plasma radio frequency instrument pig tongue radiofrequency operation, take energy level 6, the role of time 10s. The treated tongue base mass was subjected to continuous cryosectioning. One 200 μm section was reserved at intervals of 200 μm. After hematoxylin-eosin staining, two-dimensional images of the entire section of the tissue sections were collected for image segmentation and extraction. The extracted segmentation image is imported into the IPS image processing software to perform 3D reconstruction and volume calculation of the damaged area. RESULTS: The volume of each point of action was (359.5 ± 5.6) mm3, (364.3 ± 7.0) mm3, (363.7 ± 7.2) mm3, 354.1 ± 11.8 mm3, 349.4 ± 17.2 mm3, 353.5 ± 7.9 mm3, There was no significant difference in the volume of radiofrequency lesion at each point of tongue base (P> 0.05). CONCLUSION: In the radiofrequency treatment of tongue base tunnel, the damage volumes of different action points are the same at the same action level and action time. Low-temperature plasma radiofrequency treatment system, the role of energy concentration, tongue area limits the scope of damage in the tongue base area with high safety.