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1982年,Quillardet P等报导了一种监测环境中“致遗传毒性物质”(Genoto-xicants)的新方法,称SOS染色体试验,1985年,Oda Y等采用将PSK1002质粒导入TA1535的重建菌株作同类试验,使测试效果有进一步改善,改进后的方法称SOS/Umu试验。这一方法已被广泛应用于环境污染物生物学监测的实际工作中。 本法系一种特异性的“半乳糖苷酶诱导法”(简称Umu试验)。产生半乳糖苷酶的结构基因LacZ系与Umu C基因的操纵子相融
In 1982, Quillardet P et al. Reported on a new method for monitoring Genoto-xicants in the environment called the SOS chromosomal test. In 1985, Oda Y et al. Used the same type of reconstructed strain that introduced the PSK1002 plasmid into TA1535 Test, the test results to further improve the improved method called SOS / Umu test. This method has been widely used in the actual work of monitoring biological pollutants. This law is a specific “galactosidase induction method” (referred to as Umu test). The LacZ gene, a structural gene that produces galactosidase, melts with the operon of the Umu C gene