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目的观察绿茶对大鼠膀胱肿瘤生长和间隙连接蛋白43(Cx43)表达的影响。方法以N-丁基-N-(4-羟丁基)亚硝胺诱导形成大鼠膀胱癌动物模型。将39只成年雄性Wistar大白鼠随机分成正常对照组、膀胱癌模型组和绿茶组。24周后在麻醉下切除大鼠膀胱肿瘤组织并观察病理学改变,称量瘤体湿重并计算抑瘤率,免疫组织化学和DNA原位缺口末端标记法分别检测肿瘤微血管密度(MVD)和细胞凋亡指数(AI),半定量逆转录聚合酶链反应和Western印迹检测膀胱肿瘤组织Cx43 mRNA及其蛋白的表达水平。结果绿茶组的膀胱肿瘤癌变发生率、瘤体湿重、MVD分别为38.46%(5/13)、(4.47±1.01)g、(2977±776),均显著低于模型组83.33%(10/12)、(5.63±1.27)g、(3708±570)(X~2=0.041、t=0.018、t=0.014,P均<0.05),瘤重抑制率为20.82%;AI (22.46±2.90)%明显高于模型组(4.92±1.00)%(t=0.000,P<0.01)。绿茶组的Cx43 mRNA和蛋白的表达均极显著高于模型组(t=0.002、t=0.000,P均<0.01)。结论绿茶能上调Cx43在大鼠膀胱肿瘤组织中的表达和增强Cx43介导的间隙连接通讯功能,从而有效抑制大鼠膀胱肿瘤的生长。
Objective To observe the effect of green tea on the growth of bladder tumor and the expression of connexin 43 (Cx43) in rats. Methods Rat model of bladder cancer was induced by N-butyl-N- (4-hydroxybutyl) nitrosamine. Thirty-nine adult male Wistar rats were randomly divided into normal control group, bladder cancer model group and green tea group. Twenty-four weeks later, the bladder tumor tissues were removed under anesthesia and pathological changes were observed. The wet weight of the tumor was measured and the tumor inhibition rate was calculated. Immunohistochemistry and DNA in situ nick end labeling were used to detect the tumor microvessel density (MVD) and Cell apoptosis index (AI), semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting were used to detect the expression of Cx43 mRNA and protein in bladder tumor. Results The incidence of bladder cancer and MVD in green tea group were 38.46% (5/13), (4.47 ± 1.01) g and (2977 ± 776) respectively, which were significantly lower than those in model group (83.33%, 10 / The tumor weight inhibition rate was 20.82%, AI (22.46 ± 2.90), (P <0.05) % Was significantly higher than that in model group (4.92 ± 1.00)% (t = 0.000, P <0.01). The expression of Cx43 mRNA and protein in green tea group was significantly higher than that in model group (t = 0.002, t = 0.000, P <0.01). Conclusion Green tea can up-regulate the expression of Cx43 in rat bladder tumor and increase the Cx43-mediated gap junctional communication, thus effectively inhibiting the growth of rat bladder tumor.