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目的应用核磁共振成像(MRI)技术,观察蕲蛇酶(Acutobin)对高血糖大鼠脑损伤体积和灌注不足的影响。方法在高血糖大鼠右脑中动脉阻塞/再灌注(MCAO/R)的动物模型上,在MCAO60min和再灌注后24h各静注2.5U.kg-1体重蕲蛇酶1次,在MCAO/R前和后,连续检测记录多断层扩散加权成像(multslice diffusion weighted imaging,DWI)和单一断层动态快速注射轨迹梯度回波成像(single slice dynamic bolus tracking gradien echo imaging,GE)。结果蕲蛇酶治疗组DWI所检测的损伤体积,在再灌注后明显减少(P<0.05),右脑半球脑血流残留量(residual CBF)明显增多,动物死亡率明显降低。结论蕲蛇酶能改善高血糖大鼠MCAO/R模型脑组织的灌流,减少梗塞面积,降低死亡率。
Objective To observe the effect of Acutobin on brain injury volume and hypoperfusion in rats with hyperglycemia by using magnetic resonance imaging (MRI). Methods In animal models of middle cerebral artery occlusion / reperfusion (MCAO / R) in hyperglycemic rats, 2.5U.kg-1 body weight viper enzyme was injected intravenously once every 60 minutes after MCAO and 24 hours after reperfusion, Continuous recording and recording of multslice diffusion weighted imaging (DWI) and single slice dynamic bolus tracking gradien echo imaging (GE) were performed before and after R, respectively. Results The volume of injury detected by DWI in Acutus decreased significantly after reperfusion (P <0.05), while residual CBF in the right hemisphere increased significantly and animal mortality decreased significantly. Conclusion Acutobin can improve perfusion of brain tissue of MCAO / R model rats with hyperglycemia, reduce infarct size and reduce mortality.