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为了建立和优化青蒿的组织培养再生体系,获得快速繁殖技术,以青蒿叶片和叶柄为外植体,研究了不同激素配比对其离体培养和大量快速繁殖的影响。结果表明:以青蒿叶片为外植体,其愈伤组织诱导培养基为MS+2.0mg/L 6-BA+0.5mg/L 2,4-D,诱导率为78.0%;分化培养基为MS+1.0mg/L 6-BA+0.05mg/L NAA+3%蔗糖+0.7%琼脂粉,分化率达95.3%。分化苗在1/2MS+1.0mg/L NAA培养基上快速生根,且根系发达,粗壮,生根率达98.9%。以青蒿叶柄为外殖体,叶柄可在培养基MS+2.0mg/L 6-BA+0.5mg/L 2,4-D上一次成苗,在1/2MS+1.0mg/L NAA培养基上生根良好。该体系有效的缩短了培养周期,具有重要的研究价值。
In order to establish and optimize the tissue culture regeneration system of Artemisia annua and obtain the rapid propagation technology, the effects of different hormone combinations on the in vitro culture and rapid multiplication were studied using leaves and petioles of A. annua as explants. The results showed that the induction rate of callus was (MS + 2.0mg / L 6-BA + 0.5mg / L 2,4-D) and the induction rate was 78.0% MS + 1.0mg / L 6-BA + 0.05mg / L NAA + 3% sucrose + 0.7% agarose, the differentiation rate was 95.3%. Differentiated shoots rapidly rooted on 1 / 2MS + 1.0mg / L NAA medium, and the root system developed, sturdy and the rate of rooting reached 98.9%. Artemisia annua petiole as explants, petiole in the medium MS + 2.0mg / L 6-BA + 0.5mg / L 2,4-D on a seedling, in 1 / 2MS + 1.0mg / L NAA medium Good rooting. The system effectively shortens the incubation period and has important research value.