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目的探讨青海地区回族胃癌患者人群中血管紧张素转换酶(ACE)基因第16内含子的多态性分布,与疾病易感性关系及等位基因D和I分布特点。方法利用PCR技术检测回族正常和胃癌患者人群ACE基因多态性,并进行比较。结果回族胃癌患者中ACE基因三种不同基因型(II、DI和DD)频率分别为24.7%、42.0%、33.3%,而在正常对照组中分别为26.9%、30.2%、42.9%。在对照组和胃癌患者中,三种基因型没有明显差异(P>0.05)。等位基因D和I的基因频率分别为对照组0.579、0.421和患者组0.543、0.457,没有显著差别(P>0.05)。结论ACE基因D/I多态性三种基因型II、DI、DD在青海省世居回族胃癌中没有明显差别,且与胃癌易感性无关。
Objective To investigate the polymorphism distribution of angiotensin converting enzyme (ACE) gene intron 16 and its relationship with susceptibility to disease and the distribution of allele D and I in the population of Hui patients with gastric cancer in Qinghai. Methods Polymerase chain reaction (PCR) was used to detect ACE gene polymorphism in normal and gastric cancer patients. Results The frequencies of three different genotypes (II, DI and DD) of ACE gene were 24.7%, 42.0% and 33.3% respectively in patients with gastric cancer of Hui ethnicity, while they were 26.9%, 30.2% and 42.9% respectively in the normal control group. Among the control group and gastric cancer patients, there was no significant difference among the three genotypes (P> 0.05). The allele frequencies of D and I genes were 0.579 and 0.421 in the control group and 0.543 and 0.457 in the patient group respectively, with no significant difference (P> 0.05). Conclusion There are no significant differences in ACE genotype D / I polymorphism between the three genotypes II, DI and DD in the living Hui ethnic group in Qinghai Province, and not related to the susceptibility to gastric cancer.