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目的 调查TTV阴性的非甲～庚型肝炎患者中TTV -likeminivirus(TLMV)感染情况 ,对TLMV5′非编码区 (5′NCR)部分基因进行分子克隆与序列分析。方法 采用巢式PCR技术检测5 3例TTV阴性的非甲～庚肝炎患者血清TLMVDNA ,对PCR产物进行克隆、测序和分析。结果 5 3例病例中TLMVDNA阳性 37例 (6 9 8% ) ,对其中 8株TLMV基因克隆测序 ,并与Takahashi报道的TLMV序列 (GenBankAccessionNo ab 0 2 6 930、ab0 2 6 931)比较 ,其核苷酸序列同源性在 6 4%～ 83%之间。结论 在TTV阴性的非甲～庚肝炎患者中存在TLMV感染。TLMV5′NCR基因变异性较大。TLMV的致病性及其与非甲～庚肝炎的关系尚有待进一步研究
Objective To investigate the infection of TTV-like minivirus (TLMV) in TTV-negative patients with non-A ~ G hepatitis and to clone and sequence the partial 5 ’non-coding region (TLR) gene of TLMV. Methods The nested PCR technique was used to detect serum TLMV DNA in 53 TTV-negative patients with non-A-G hepatitis and the PCR products were cloned, sequenced and analyzed. Results Totally 37 TLMV DNA positive cases (69.8%) were obtained in 5 of 3 cases. 8 TLMV genes were cloned and sequenced. Compared with the TLMV sequence (GenBank Accession No. ab 0 2 6 930, ab0 2 6 931) reported by Takahashi, The nucleotide sequence homology is between 64% and 83%. Conclusions There is a TLMV infection in TTV-negative patients with non-A-G hepatitis. TLMV5’NCR gene variability larger. The pathogenicity of TLMV and its relationship with non-hepatitis A ~ G remains to be further studied