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用全细胞膜片钳技术首次研究了成年小鼠单个心室肌细胞内向整流钾电流(I_(k1))的整流特性,膜电导和反转电位对胞外钾离子浓度的敏感性及激活、失活动力学等性质。发现小鼠心室肌细胞I_(k1)最突出的特点就是其电压-电流(I～V)曲线在正于反转电位50mV的范围内无负斜率(negative slope)区,而是保持在一个很低的外向电流水平(59±39)pA。当用3mmol/L K~±和3mmol/L Cs~±灌流时,这一部分的I_(k1)减小为零,随后产生早后去极化(EAD)。结果提示:小鼠I_(k1)的这一特点与其EAD易发有关,I_(k1)受抑是本实验EAD发生的先决条件。
Whole-cell patch-clamp technique was used to study the rectifying characteristics of inward rectifier potassium current (I_ (k1)) in adult mouse single ventricular myocytes. Sensitivity and activation of membrane conductivity and reversal potential to extracellular potassium concentration, Mechanics and other properties. It is found that the most prominent feature of I_ (k1) in mouse ventricular myocytes is that the voltage-current (I ~ V) curve has no negative slope within the range of 50 mV against the reversal potential, Low outward current level (59 ± 39) pA. This fraction decreases I_ (k1) to zero when primed with 3 mmol / L K ~ ± and 3 mmol / L Cs ~ ± and subsequently produces an early depolarization (EAD). The results suggest that this characteristic of mouse I_ (k1) is related to the development of EAD, and inhibition of I_ (k1) is a prerequisite for the occurrence of EAD in this experiment.