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目的 观察异丙酚对急性吗啡耐受小鼠痛阈的影响.方法 健康雌性小鼠56只,随机分成7组(n=8):吗啡异丙酚组1(MP1)、吗啡异丙酚组2(MP2)、吗啡异丙酚组3(MP3)、大豆油对照组(SC)、生理盐水异丙酚组1(NSP1)、生理盐水异丙酚组2(NSP2)、生理盐水异丙酚组3(NSP3).通过皮下注射吗啡建立急性吗啡耐受模型:MP1、MP2、MP3组和SC组小鼠皮下注射吗啡10 mg/kg,2h一次,连续注射5次.建模成功后各组分别给予异丙酚10、30、50 mg/kg和注射用大豆油10μl.NSP1、NSP2、NSP3组小鼠皮下注射生理盐水10 mg/kg后腹腔给予异丙酚(NSP1组10 mg/kg、NSP2组30 mg/kg、NSP3组50 mg/kg).在各次皮下注射吗啡及腹腔注射异丙酚溶液后10、30、60、120 min分别用热板法测量热缩足反应时间作为小鼠的热痛阈值.结果 吗啡异丙酚组:与SC组相比,MP1组热痛阈值的差异无统计学意义(P＞0.05),MP2组和MP3组小鼠热痛阈值提高(P＜0.05);MP2组、MP3组与MP1组相比热痛阈值提高(P＜0.05);MP2组与MP3组小鼠热痛阈值的差异无统计学意义(P＞0.05).生理盐水异丙酚组:注射生理盐水前后,小鼠热痛阈值的差异无统计学意义(P＞0.05);腹腔注射异丙酚后,NP1、NP2、NP3组小鼠热痛阈值的差异无统计学意义(P＞005).结论 异丙酚对正常小鼠的热痛阈值无影响;异丙酚30 mg/kg和50 mg/kg能够使急性吗啡耐受和痛觉过敏的小鼠热痛阈值提高,拮抗吗啡镇痛耐受与痛觉过敏,但异丙酚10 mg/kg拮抗作用不明显.“,”Objective To observe the effects of propofol on the pain threshold of mice with acute morphine tolerance.Methods A total of 56 healthy female mice were randomly divided into seven groups (n =8):Groups morphine and propofol 1,2 and 3 (MP1,MP2 and MP3),Group soybean oil control (SC) and Groups normal saline and propofol 1,2 and 3 (NSP 1,2 and 3).A model of acute morphine tolerance was established through subcutaneous injection of morphine,where Groups MP 1,2 and 3 and Group SC were subcutaneously injected with 10 mg/kg morphine once every two hours for five consecutive times,followed by administration of 10 mg/kg propofol (Group MP 1),30 mg/kg propofol (Group MP 2),50 mg/kg(Group MP 3) as well as 10 μ1 soybean oil (Group SC).In contrast,Groups NSP1,NSP2 and NSP3 were subcutaneously injected with 10 mg/kg normal saline followed by administration of 10 mg/kg propofol (Group NSP 1),30 mg/kg propofol (Group NSP 2),and 50 mg/kg (Group NSP 3).The paw withdrawal thermal latency was measured as the hot-pain thresholds of mice 10,30,60 and 120 min after administration of propofol solution.Results Compared with Group SC,no significant difference was found in the hot-pain threshold of Group MP 1 (P ＞ 0.05),while increased hot-pain thresholds were observed in Groups MP 2 and 3 (P ＜ 0.05).Furthermore,compared with Group MP 1,both Groups MP 2 and 3 presented higher hot-pain thresholds (P ＜ 0.05).The hot-pain thresholds were no significantly different between Groups MP 2 and 3 (P ＞ 0.05).Meanwhile,no statistical difference was found as to hot-pain threshold before and after injection of normal saline (P ＞ 0.05).No statistical differences were found among Groups NSP1,NSP2 and NSP3 after subcutaneous injection of propofol (P ＞ 0.05).Conclusions Propofol produces no impact on the hot-pain threshold of normal mice.Administration of 30 mg/kg and 50 mg/kg propofol can enhance the hot-pain threshold of mice with acute morphine tolerance and hyperalgesia.However,10mg/kg of propofol can not produce obvious antagonist effects.