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目的:考察淫羊藿总黄酮苷在家兔肠菌中的代谢以及葡萄糖酸内酯对肠菌代谢的抑制作用,旨在帮助揭示淫羊藿总黄酮苷在家兔体内的代谢途径和预防激素性骨坏死作用的物质基础。方法:淫羊藿总黄酮苷与家兔肠菌液经37℃厌氧温孵培养后,在不同时间点经水饱和的乙酸乙酯萃取,并对加入不同浓度(8,12和16mg·mL-1)的葡萄糖酸内酯对淫羊藿总黄酮苷经家兔肠菌代谢的抑制情况进行考察。采用ODS柱和乙腈-水(各含0.1%的甲酸)的流动相梯度洗脱,检测波长为335nm。结果:淫羊藿总黄酮苷在家兔肠菌液中温孵2h时被代谢成淫羊藿次苷Ⅱ,在温孵8h时被代谢成淫羊藿素;葡萄糖酸内酯能够浓度依赖性地抑制家兔肠菌对淫羊藿总黄酮苷的代谢。结论:淫羊藿总黄酮苷能被家兔肠道菌群迅速水解成淫羊藿次苷Ⅱ和淫羊藿素;当葡萄糖酸内酯的浓度为16mg·mL-1时,则能完全抑制家兔肠菌对淫羊藿总黄酮苷的代谢。
OBJECTIVE: To investigate the metabolism of total flavonoid glycosides of Herba Epimedii in E. coli and the inhibitory effect of gluconolactone on the metabolism of enteric bacteria, aiming at revealing the metabolic pathway and preventive hormone of epimedium glycosides in rabbits The material basis of the role of osteonecrosis. Methods: Total flavonoid glycosides of Epimedium breviscapus and enteric-coated intestinal fluid of rabbits were cultured in anaerobic condition at 37 ℃ and then extracted with ethyl acetate saturated with water at different time points. After adding different concentrations (8, 12 and 16 mg · mL -1) of gluconolactone on Epimedium total flavonol glycosides by the inhibition of intestinal metabolism in rabbits were investigated. The mobile phase was eluted using an ODS column and acetonitrile-water (each containing 0.1% formic acid) at a detection wavelength of 335 nm. Results: The total flavonoid glycosides of Epimedium brevicornis were metabolized to icaritidine Ⅱ when incubated in the intestinal fluid of rabbits for 2 hours and metabolized to icarubicin at 8 hours after incubation. Gluconolactone could be metabolized in a concentration-dependent manner Inhibition of Enterobacter aggression Herba epimedium flavonoid glycosides metabolism. Conclusion: The total flavonoid glycosides of epimedium can be quickly hydrolyzed into icaritin Ⅱ and icaritin by the intestinal flora of rabbits. When gluconolactone concentration is 16 mg · mL-1, it can be completely inhibited Metabolism of Total Flavone Glycosides of Herba Epimedii by Enterobacter.