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目的探讨Wnt通路组成部分糖原合成酶激酶3β(glycogen synthase kinase 3β,GSK-3β)基因rs3755557位点及β连环蛋白(β-catenin)基因rs1880481位点多态性与胃癌发病风险的关系。方法经PCR扩增后,以变性高效液相色谱结合DNA自动测序分析了26例胃癌患者和33例慢性浅表性胃炎患者(对照组)rs3755557位点及rs1880481位点基因型及等位基因频率分布的差异。结果统计学分析发现rs3755557位点基因型及等位基因频率在胃癌组与对照组间差异无统计学意义。rs1880481位点等位基因频率在各胃癌组与相应对照组间差异无统计学意义。该位点杂合基因型在男性对照组的频率(68.18%)显著高于男性胃癌组(26.67%),OR=5.893,95%CI1.377～25.226(P=0.013);而该位点的AA基因型在男性对照组和男性胃癌组中的频率则分别为9.09%和40.00%,两者差异有统计学意义,OR=6.667,95%CI1.121～39·660(P=0.025)。结论GSK-3β基因rs3755557位点基因型及等位基因不增加胃癌发病风险。β-catenin基因rs1880481位点杂合基因型低下或者AA基因型升高可能导致胃癌发生的风险性增加。
Objective To investigate the relationship between the rs3755557 site of glycogen synthase kinase 3β (GSK-3β) gene and the rs1880481 site of β-catenin gene and the risk of gastric cancer in Wnt pathway. Methods The genotype and allele frequencies of rs3755557 and rs1880481 in 26 patients with gastric cancer and 33 patients with chronic superficial gastritis (control group) were analyzed by denaturing high performance liquid chromatography coupled with DNA sequencing after PCR amplification. Differences in distribution. Results Statistical analysis found no significant difference in genotype and allele frequencies of rs3755557 between gastric cancer group and control group. rs1880481 allele frequency in each gastric cancer group and the corresponding control group no significant difference. The frequency of heterozygous genotypes in the control group (68.18%) was significantly higher than that in the male patients (26.67%), OR = 5.893,95% CI1.377 ~ 25.226 (P = 0.013) The frequencies of AA genotypes in male control group and male gastric cancer group were 9.09% and 40.00%, respectively. The difference was statistically significant (OR = 6.667, 95% CI 1.121-39.660, P = 0.025). Conclusion Genotypes and alleles of rs3755557 GSK-3β gene do not increase the risk of gastric cancer. The heterozygous genotype of rs1880481 in β-catenin gene or AA genotype may increase the risk of gastric cancer.