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利用PCR和限制性内切酶分析,探讨了EB病毒基因组限制片段长度多态型(RFLP)在正常鼻咽组织,鼻咽癌组织和口腔上皮脱落细胞中的分布情况。结果表明在正常鼻咽组织的EB病毒感染是一种普遍现象并且感染可能发生在癌变前;EB病毒基因组BamHF区的RFLP在鼻咽癌组织和正常的鼻咽组织之间有不同的分布;不同EB病毒RFLP变型(variant)的混合感染在正常咽组织比在癌组织中普遍,这结果表明可能在癌变发展过程中,由于部分带有EB病毒的细胞的选择生长,结果使得癌组织中的细胞以一种EB病毒变型为主;本文探讨了EB病毒的RFLP变型在鼻咽组织和口腔上皮脱落细胞分布的关系,这种关系可能为鼻咽癌高发区的癌变检测提供一种简单可靠的方法。
The distribution of restriction fragment length polymorphism (RFLP) of Epstein-Barr virus in normal nasopharyngeal tissues, nasopharyngeal carcinoma and oral epithelial exfoliated cells was investigated by PCR and restriction endonuclease analysis. The results show that EBV infection in normal nasopharyngeal tissues is a common phenomenon and the infection may occur before carcinogenesis; RFLP in EBV genome BamHF region has different distribution between nasopharyngeal carcinoma and normal nasopharyngeal tissues; different The mixed infection of Epstein-Barr virus (RFLP) variants is more common in normal pharyngeal tissues than in cancerous tissues. This result suggests that the selective growth of some Epstein-Barr virus-bearing cells during carcinogenesis may result in cells in cancerous tissues A variant of Epstein-Barr virus (EBV); the relationship between the RFLP variants of Epstein-Barr virus (EBV) variants and the distribution of oral epithelial exfoliative cells in nasopharyngeal tissues was explored in this paper. This relationship may provide a simple and reliable method for the detection of cancerous lesions in high- .