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目的研究江西省部分医院临床分离的致泻沙门菌耐药性与分子分型,为临床科学选择抗菌药物提供参考。方法采用微量肉汤稀释法和基因检测技术,对江西省部分医院临床分离的腹泻性沙门菌进行药敏试验和基因分析。结果从医院住院感染性腹泻患者送检标本中分离出沙门菌103株,对四环素耐药率达到61.17%,有27.18%的菌株呈现多重耐药性。该省多数地区医院分离的沙门菌对磺胺类、四环素类、头孢类抗菌药物耐药率均有上升趋势,对喹诺酮类抗菌药物较敏感。从2015年分离的菌株基因分型检测发现52种带型,每种带型包含菌株为1~3株,相似度为0%~100%。结论该省医院分离的腹泻感染沙门菌耐药状况比较严重,菌株PFGE带型呈现多样性,提示应加强抗菌药物管理。
Objective To study the drug resistance and molecular typing of clinically isolated Salmonella enteritidis in some hospitals of Jiangxi province and to provide a reference for selecting antibiotics in clinical science. Methods The antibiotic susceptibility test and gene analysis of clinically isolated diarrhea Salmonella in some hospitals of Jiangxi Province were carried out by using the method of micro broth dilution and gene detection. Results 103 strains of Salmonella were isolated from the hospitalized inpatients with infectious diarrhea, and the rate of resistance to tetracycline was 61.17%. 27.18% strains showed multi-drug resistance. Salmonella isolated from hospitals in most areas of the province showed an upward trend in resistance rates to sulfonamides, tetracyclines and cephalosporins, and were more sensitive to quinolone antibiotics. Genotypes of strains isolated from 2015 found 52 bands, each band containing 1 to 3 strains, the similarity was 0% to 100%. Conclusions The Salmonella resistance of diarrhea-infected diarrhea in the provincial hospital is rather serious. The diversity of PFGE bands in the strains shows diversity, suggesting that the management of antibiotics should be strengthened.